Skin Tissue Engineering

The chapters in this book cover topics such as Isotopic Ratio Outlier Analysis (IROA) for quantitative analysis; cholesterol and derivatives in ocular tissues using LC-MS/MS methods; microbial siderophores analysis by mass spectrometry; the metabolomic study of tissues in Parkinson’s Disease; and NMR analysis in livestock metabolomics.Written in the highly successful Methods in Molecular Biology series format, the chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step readily reproducible laboratory protocols, tips on troubleshooting and avoiding pitfalls. The volume brings together some of the best experts in the field of modern metabolomics to discuss various techniques used today to study specific metabolite classes, and metabolomics in bacterial systems and mammalian systems.

Written in the highly successful Methods in Molecular Biology series format, the chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step readily reproducible laboratory protocols, tips on troubleshooting and avoiding pitfalls. Metabolomics.

Microbial Lipid Production Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

The protocols presented in the book consider sugars derived from different substrates including chemically pre-treated agricultural residues, industrial residues containing lignin, food wastes, and industrial waste water in an approachable format.. Comprehensive and cutting-edge, Microbial Lipid Production: Methods and Protocols is a valuable resource aimed at novice researchers who are working on improving their knowledge and skills in this developing field.Microbial Lipid Production Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

The book also discusses applications that use oleaginous organisms to transform substrates into a variety of products including bio-crude, high value fatty acids, biofuels such as biodiesel, neutral lipids, volatile fatty acids, and surfactants. The protocols presented in the book consider sugars derived from different substrates including chemically pre-treated agricultural residues, industrial residues containing lignin, food wastes, and industrial waste water in an approachable format. Comprehensive and cutting-edge, Microbial Lipid Production: Methods and Protocols is a valuable resource aimed at novice researchers who are working on improving their knowledge and skills in this developing field..

Chapters in this book cover topics such as requirements and preconditions for using human induced pluripotent cell lines in assay development; the culture of iPSCs and iPSC-derived cardiomyocytes by bioreactor technologies; distinctions between hepatocytes, vascular endothelial cells, cardiomyocytes, renal podocytes, and neurons; and high content assays of mitochondrial function and autophagy. Cell-Based Assays Using iPSCs for Drug Development and Testing.Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. The volume presents a variety of protocols that will be useful to researchers working in the field of disease modeling, drug discovery, and the cell biology of various tissues.

This volume provides protocols describing the isolation and culture of diverse cell types stemming from the skin and the use of these cells and cell constructs for wound healing, bioengineering applications, and translational medicine purposes. Skin Tissue Engineering.Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. The book is divided into three sections describing the isolation and culture of diverse skin cells, managing these cells within co-culture systems and skin models, as well as using these skin models in a test setting.

Cell-Based Assays Using iPSCs for Drug Development and Testing. Chapters in this book cover topics such as requirements and preconditions for using human induced pluripotent cell lines in assay development; the culture of iPSCs and iPSC-derived cardiomyocytes by bioreactor technologies; distinctions between hepatocytes, vascular endothelial cells, cardiomyocytes, renal podocytes, and neurons; and high content assays of mitochondrial function and autophagy. The volume presents a variety of protocols that will be useful to researchers working in the field of disease modeling, drug discovery, and the cell biology of various tissues.

This volume provides protocols describing the isolation and culture of diverse cell types stemming from the skin and the use of these cells and cell constructs for wound healing, bioengineering applications, and translational medicine purposes.

Chapters in this book cover topics such as requirements and preconditions for using human induced pluripotent cell lines in assay development; the culture of iPSCs and iPSC-derived cardiomyocytes by bioreactor technologies; distinctions between hepatocytes, vascular endothelial cells, cardiomyocytes, renal podocytes, and neurons; and high content assays of mitochondrial function and autophagy.