Bacterial Chromatin

The chapters in this book cover topics, such as next-generation-flow-based methods for detection of minimal residual disease and circulating tumor cells; Cytometry by Time-of-Flights (CyTOF); a droplet digital PCR approach for finding contamination in patients’ samples; genome-wide investigation of the methylation and hydroxymethylation status of cytosines; and a microfluidic approach for creating nanoparticles.Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. This volume provides a thorough guide of the study of multiple myeloma (MM)—from sample acquisition to the performance of molecular tests, and also discusses difficulties that arise during quality control and interpretation of results.

Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cancer Epigenetics for Precision Medicine.

This volume presents methods and protocols on modern analysis of steroid receptor and prostate cancer function.

Chapters guide readers through the production of VNPs derived from plant, animal and bacterial viruses, prokaryotic and eukaryotic expression systems, encapsulation of heterologous materials within VNPs, and the modification of the outer surface of VNPs and how such modified VNPs can be developed into functional entities.Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. This volume details protocols on virus-derived nanoparticles (VNPs) for a number of different applications. Virus-Derived Nanoparticles for Advanced Technologies.

The chapters in this book cover topics such as coomassie-brilliant blue staining of polyacrylamide gels; silver staining techniques; microwave assisted protein staining, de-staining, and in-solution digestion of proteins; curumin and turmeric as an environment-friendly protein gel stain; in-gel protein phosphotase assay using fluorogenic substrates; destaining with fungal laccase; and radiolabeling and analysis of labeled proteins. This volume expands upon the collection of techniques published in Protein Electrophoresis: Methods and Protocols (2012) with more practical and reproducible methods to study protein gel detection and imaging.Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Bacterial Chromatin. Chapters detail experimental protocols of in vivo and in vitro approaches, approaches to genome structure modeling, and data analysis. This volume brings together a wide range of methods to explore the structure and function of bacterial chromatin from molecular to the cellular scale.

The chapters in this book cover topics such as crystallography and phage display, peptide design, phospholipid binding, and thrombus formation to microbiome analysis and development. This volume explores the latest methods used to study and define serpin molecular structure, basic protease inhibition, serpin targets, and the roles of serpin in biology and disease using animal models.

Chapters detail experimental protocols of in vivo and in vitro approaches, approaches to genome structure modeling, and data analysis.

The chapters in this book cover topics such as crystallography and phage display, peptide design, phospholipid binding, and thrombus formation to microbiome analysis and development.Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.