Comparative Embryo Culture

The final section addresses more general protocols including plasmid transfer and bioinformatic tools as well as novel applications of transposon methodologies such as transposon-aided capture of antibiotic resistant plasmids..Microbial Transposon Mutagenesis Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

The species included in this volume represent a broad range of taxa, whose embryos have vastly different culture requirements and developmental characteristics.Comparative Embryo Culture Written for the highly successful Methods in Molecular Biology series, chapters include introduction to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls. Among the species described in this volume, significant differences exist in the rates of cell division, the timing of the maternal to embryonic transition, the relative lipid content of the cytoplasm, the sensitivity of the embryo to specific environmental ions, the preferred nutrients and associated metabolic pathways used by the embryo, the timing and mechanisms of early lineage specification, the presence or absence of embryonic diapause, and the time from fertilization to implantation.

The volume begins with methods for specific microorganisms and include protocols for individual microorganisms ranging from pathogens such as Salmonella to Bifidobacterium, a microorganism considered beneficial to humans and animals.Microbial Transposon Mutagenesis Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Microbial Transposon Mutagenesis Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

The volume begins with methods for specific microorganisms and include protocols for individual microorganisms ranging from pathogens such as Salmonella to Bifidobacterium, a microorganism considered beneficial to humans and animals.